How to Reconstitute
Lyophilized Peptides
A complete walkthrough for safely preparing your peptides for use. Follow these steps carefully to maintain potency and ensure sterility.
Visual Overview
Reference infographic — scroll down for detailed instructions for each step
What You'll Need
Before starting, gather all supplies and set up a clean workspace. Having everything ready prevents contamination from pausing mid-process to find materials.
Step-by-Step Instructions
Click each step to expand for detailed instructions, tips, and important warnings.
Do's & Don'ts
Do
- Use bacteriostatic water for multi-use vials
- Sanitize vial tops before every use
- Add water slowly along the glass wall
- Store reconstituted peptides in the refrigerator
- Use a new syringe for every injection
- Gently swirl to dissolve — be patient
- Keep peptides away from light and heat
- Check expiration dates before use
Don't
- Never shake or vigorously agitate the vial
- Never spray water directly onto the powder
- Never freeze reconstituted peptides
- Never reuse syringes or needles
- Never touch the rubber stopper after sanitizing
- Never use a cloudy or discolored solution
- Never store at room temperature for extended periods
- Never mix different peptides in the same vial
Frequently Asked Questions
Common questions about peptide reconstitution, storage, and handling.
Understanding Syringe Sizes
Choosing the correct syringe and needle gauge is essential for accurate dosing and comfortable injections. Different compounds and injection methods require different equipment.
Subcutaneous (Sub-Q) Syringes
Used for injecting into the fatty tissue just below the skin. This is the most common method for peptide administration.
- 1ml / 100 unit insulin syringe (29-31 gauge)
Most peptides (BPC-157, TB-500, GHRPs, GLP-1s)
- 0.5ml / 50 unit insulin syringe (30-31 gauge)
Small-dose peptides, microdosing protocols
- 0.3ml / 30 unit insulin syringe (31 gauge)
Very small doses, pediatric or sensitive areas
Intramuscular (IM) Syringes
Used for injecting directly into muscle tissue. Required for certain compounds that need deeper tissue absorption.
- 3ml syringe + 25 gauge 1" needle (25 gauge)
Testosterone, HCG, B12, larger volume injections
- 3ml syringe + 23 gauge 1.5" needle (23 gauge)
Thicker oil-based compounds, gluteal injections
- 1ml syringe + 27 gauge 0.5" needle (27 gauge)
Deltoid IM injections, smaller volumes
Understanding Needle Gauge
The gauge number refers to the thickness of the needle. Higher gauge = thinner needle. A 31-gauge needle is much thinner (and less painful) than a 23-gauge needle. Here's a quick reference:
Sub-Q Injection Sites
- Abdomen (2 inches from navel) — most common, best absorption
- Upper outer thigh — easy self-injection
- Back of upper arm — good for smaller volumes
- Rotate sites with each injection to prevent lipodystrophy
- Pinch skin, insert at 45-90° angle depending on body fat
IM Injection Sites
- Deltoid (upper arm) — for volumes under 2ml
- Vastus lateralis (outer thigh) — easy self-injection
- Ventrogluteal (hip) — preferred for larger volumes
- Dorsogluteal (upper buttock) — traditional site, use with caution
- Insert needle at 90° angle, aspirate briefly, inject slowly
Ready to Calculate Your Dose?
Use our Dosing Calculator to determine exactly how much BAC water to add and how many units to draw for your specific peptide and desired dose.